Effect of hyperbaric oxygen on an endothelin-1-induced focal cerebral ischemic model in rats
- Author:
Guo-Jun GU
1
Author Information
1. Department of Diving Medicine
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(10):1134-1137
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a focal cerebral ischemia/reperfusion (FCI/R) model with endothelin-1 (ET-1) in rats and to observe the effectiveness of hyperbaric oxygen (HBO) in treatment of this model. Methods: Three μl ET-1 (60 pmol per μl) was injected into the site adjacent to rat middle cerebral artery (MCA) under anesthesia to induce FCI/R model. HBO (100% O2, 2.5 ATA for 2 h) was applied at 1 h after ET-1 injection in ET-1+HBO group; rats in FCI/R model group received no treatment. Neurological scores were determined at 4 h, 8 h, and 24 h after injection. Animals were all killed at 24 h to harvest brain tissues for water content assessment and TTC, H-E staining. Results: ET-1 had a high successful rate in inducing FCI/R model in rats, with stable infraction area and good repeatability. Animals in ET-1+HBO group had better neurological outcomes than controls at all the 3 time points (P<0.05 at 4 h and 8 h, P<0.01 at 24 h). HBO treatment significantly decreased the brain water content (P<0.01), reduced the infarction areas (P<0.05), and obviously alleviated the cerebral lesions as showed by H-E staining. Conclusion: The ET-1-induced FCI/R model provides an ideal model for studying human cerebral ischemia diseases. HBO (100% O2, 2.5 ATA for 2 h) treatment administrated 1 h after focal cerebral ischemia can improve the neurological outcomes of the model animals, reduce the infarction areas, and alleviate ischemic brain lesion.
