Construction of citrostatin and its activity analysis
- Author:
Li MA
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(11):1249-1253
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct a new recombinant anti-tumor peptide Citrostatin, which consists of 2 anti-tumor domains (by different mechanism), and to study its biological activity, in an effort to search for a peptide with double anti-tumor efficiency. Methods: Citrostatin, a 48-amino acid peptide, was artificially synthesized. After purified by HPLC, Citrostain were subjected to the following tests separately: inhibition of endothelial cell proliferation, MTT test of cytotoxicity, inhibition of endothelial cell tube formation on ECMatrix™, and anti-angiogenesis test in chick chorioallantoic membrane. Results: Citrostatin significantly inhibited the proliferation of human endothelial cell ECV304 (ED50 = 6.2 μg/ml, P<0.05). It also significantly inhibited the proliferation of human tumor cells 1990 and NCI-H640 (ED50 = 50 μg/ml, 16 tLg/ml, both P<0.05), and the inhibitory effect became more marked (P<0.01) with the increase of Citrostatin concentration. The inhibitory effects of Citrostatin on endothelial cell tube formation and angiogenesis in chick chorioallantoic membrane were also confirmed. Conclusion: An anti-tumor 48-amino acid peptide, Citrostatin, has been successfully synthesized and purified, which shows an anti-angiogenesis effect and a direct cytotoxic effect toward tumor cells.
