Establishment of a βB2 crystallin gene knockout mice model
- Author:
Jun ZHANG
1
Author Information
1. Central Laboratory
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(11):1246-1248
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a βB2 crystallin gene knockout mice model. Methods: The gene-targeting vector was established by the technique of American in Genious Targeting Laboratory. PCR technique was used to identify the genotype of the model mice and expression of βB2 crystallin protein was detected by Western blot. Results: Three genotypes were successfully identified and expression of βB2 crystallin protein was not detected in gene knockout mice. Conclusion: The PB2 crystallin gene knockout mice model has been successfully established.
