Homocysteine accelerates senescence of cultured bovine aortic endothelial cells
- Author:
Xiao-Li CHEN
1
Author Information
1. Baoshan Branch of Shanghai Food and Drug Administration
- Publication Type:Journal Article
- Keywords:
Cell aging;
Endothelial cells;
Homocysteine
- From:
Academic Journal of Second Military Medical University
2010;28(2):162-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of homocysteine (HCY) in promoting senescence of cultured endothelial cells (EC) and the underlying mechanism. Methods: Bovine aortic endothelial cells were isolated by enzymatic digestion method from new born calf and were randomly divided into 4 groups. Cells in control group were not treated with HCY and those in the other 3 groups were treated with HCY with the final concentrations being 0.1, 0.5, and 1.0 mmol/L. The cultured cells were observed morphologically and stained with β-Gal; the cell cycle was examined with flow cytometric method (FCM) and the telomere length was analyzed by Southern blotting. Meanwhile, the contents of nitric oxide(NO), endothelin (ET), MDA and the activity of GSH-Px were determined in the supernatants. Results: Compared with control group, the 3 HCY groups had more P-Gal positive cells and shorter telomere length. Cells of G0/G1 phase in the 3 HCY groups were significantly increased than those of control group (P<0.05) and the cells of S-phase were significantly decreased (P
