In vitro antineoplastic effect of erythrocytes co-encapsulated with methotrexate and vincristine
- Author:
Xuan WANG
1
Author Information
1. Medical Information Center of PLA Jinan Military Area Command
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell cycle;
Drug carriers;
Erythrocytes;
K562 cell;
Methotrexate;
Vincristine
- From:
Academic Journal of Second Military Medical University
2010;28(2):158-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the in vitro anti-tumor effect of methotrexate (MTX) and vincristine (VCR)-loaded erythrocytes. Methods: MTX + VCR-loaded, MTX-loaded, and VCR-loaded erythrocytes were prepared by modified hypoosmotic swelling technique. K562 cells were treated with the prepared erythrocytes, pure erythrocytes, MTX+VCR solution, and RPMI 1640 medium. MTT assay was used to examine the proliferation of K562 cells. The cell cycle of K562 was observed by flow cytometer using PI dyeing. The apoptosis of co-cultured K562 cells was analyzed by flow cytorneter with Annexin V-FITC/PI kit. Results: MTX + VCR-loaded erythrocytes efficiently inhibited the proliferation and induced apoptosis of co-cultured K562 cells in a time-dependent manner, and its effects were better than those mono-loaded ones (P<0.05) and unloaded ones(P
