Inhibitory effect of small interfering RNA on migration of human colorectal cancer cell line
- Author:
Chao XU
1
Author Information
1. Department of General Surgery
- Publication Type:Journal Article
- Keywords:
Intestinal neoplasm;
Neoplasm metastasis;
Receptors, CXCR4;
RNA, small interfering
- From:
Academic Journal of Second Military Medical University
2010;28(5):470-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the inhibitory effect of small interfering RNA (siRNA) on migration of human colorectal cancer cell line. Methods: CXCR4 expression in colorectal cancer cell line SW480, SW620 and LoVo was determined by RT-PCR and Western blotting. Migratory responses of CXCR4 to stromal cell-derived factor 1 (SDF-1) were detected by cellular migration assays. The siRNA complementary to CXCR4 mRNA and its control, Non-silencing double-stranded RNA (Non-silencing dsRNA), were embedded in cationic liposome Lipofectin and transfected into SW480 cells at the final concentration of 150 nmol/L; semi-quantitative RT-PCR and Western blotting were performed to evaluate the CXCR4 mRNA and protein expression levels, respectively. The migration of transfected SW480 cells were measured quantitatively by Transwell migration assays. Results: CXCR4 expression was strong in SW480 cells, intermediate in SW620 cells, and weak in LoVo cells. In vitro cellular migration assays showed that the cell migration of SW480 and SW620 cells was more obvious than that of LoVo cells (P <0.01). The expression of CXCR4 mRNA and protein and the migration of siRNA-treated SW480 cells were significantly decreased compared with those of control group, liposome group and Non-silencing dsRNA group (P<0.01). Conclusion: CXCR4 siRNA can significantly inhibit the migration of human colorectal cancer cell line SW480 to SDF-1 by downregulating CXCR4 expression.
