Expression of AQP-1, AQP-3, AQP-4 and AQP-5 in pulmonary tissues of mice with endotoxin-induced acute lung injury
10.3724/SP.J.1008.2008.00131
- Author:
Bo LI
1
Author Information
1. Department of Respiratory Medicine
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Aquaporins;
Lipopolysaccharide;
Pulmonary edema
- From:
Academic Journal of Second Military Medical University
2010;29(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression of AQP-1, AQP-3, AQP-4 and AQP-5 in the pulmonary tissues of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) and to clarify the relationship of ALI with the expression of AQP-1, AQP-3, AQP-4 and AQP-5. Methods: Forty healthy male C57BL/6 mice were evenly randomized into 5 groups: LPS 4 h group, LPS 6 h group, LPS 8 h group, LPS 10 h group and control group. ALI model was induced with LPS in all the LPS groups. Real-time PCR was used to observe the expression changes of AQP-1, AQP-3, AQP-4 and AQP-5 mRNA. Immunohistochemical method and Western blotting assay were used to determine the changes of AQP-1, AQP-3, AQP-4 and AQP-5 protein in the pulmonary tissues of all the animals. Meanwhile, measurement of lung wet/dry (W/D) weight ratio and pathological staining were performed in each group. Results: The W/D values of the LPS 4 h, 6 h, 8 h and 10 h groups (4.39±0.19,4.58± 0.17,4.87±0.21 and 5.28±0.16, respectively) were significantly higher than that of the control group (3.99±0.25, all P<0.05). The expression of AQP-1 and AQP-5 protein in the lung of the LPS 4 h group decreased to (74.1±5.2)% and (70.3±7.1)% that of the control group; the expression of AQP-1 and AQP-5 protein in the lung of the LPS 8 h group decreased to (45.2±4.4)% and (38.6±8.9)% that of the control group, and the expression of AQP-3 and AQP-4 had no obvious changes. Conclusion: AQP-1 and AQP-5 may play important roles in the abnormal ALI fluid transportation and might be associated with the development of pulmonary edema. AQP-3 and AQP-4 may not participate in the development of pulmonary edema during ALI.
