Comparison between effects of mycophenolate mofetil and rapamycin on proliferation and apoptosis of cyst-lining epithelial cells of ADPKD patients
10.3724/SP.J.1008.2008.00921
- Author:
Tong ZHANG
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell proliferation;
Cyst-lining epithelial cell;
Mycophenolate mofetil;
Polycystic kidney disease;
Rapamycin
- From:
Academic Journal of Second Military Medical University
2010;29(8):921-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of mycophenolate mofetil(MMF)on proliferation and apoptosis of cyst-lining epithelial cells in patients with autosomal dominant polycystic kidney disease (ADPKD), and to compare its effect with that of rapamycin (RAPA) in vitro. Methods: Primary cultured cyst-lining epithelial cells were treated with MMF and RAPA at different concentrations(0, 0.005, 0.05, 0.5, 5 μg/ml)for 48 h or 72 h. The inhibitory effects of them on the cells were evaluated by MTT assay; the cell cycle distribution and apoptotic ratio were determined by flow cytometry. The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope. Results: Both MMF and RAPA significantly inhibited the proliferation of cyst-lining epithelial cells in a dose- and time dependent manner. After 48 h treatment, the cells were blocked at S phase by MMF and at G0/G1 phase by RAPA. Both drugs induced cell apoptosis, with the maximal apoptotic rate being (5.53 ± 0.27)% for MMF and (4.36 ± 0.10)% for PAPA. Typical morphological changes of apoptotic cells were observed under electron microscope. Conclusion: MMF can effectively inhibit proliferation and induce apoptosis of cyst-lining epithelial cells, but its inhibitory effect is weaker than that of RAPA.
