Effect of bone marrow mesenchymal stem cells transplantation in reconstructing hematopoiesis function of mice with critical irradiation injury
10.3724/SP.J.1008.2008.01015
- Author:
Fan-Feng LIU
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Nematopoietic system;
Radiation injuries
- From:
Academic Journal of Second Military Medical University
2010;29(9):1015-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of bone marrow mesenchymal stem cells (mMSC) infusion on the reconstruction of hematopoiesis function in mice with critical irradiation injury. Methods: Female C57BL/6 mice irradiated with 8 Gy 60 Co γ-rays were randomly divided into control group and mMSCs-treated group. Mice in the mMSCs-treated group received mMSC of male C57BL/6 mice. The peripheral leukocyte, platelet, and erythrocyte counts, the pathological lesions, granulocyte-macrophage progenitors (CFU-GM),and sex chromosome ratio were all examined. Results: All the animals in the control group (n = 6) died of hematopoiesis exhaustion 20 days after irradiation. The peripheral leukocyte of mMSC-treated group decreased greatly after bone marrow transplantation and quickly recovered 2 weeks later; till 28 days after transplantation, it recovered to 60% of the pre-irradiation level. mMSCs infusion promoted the quick recovery of the nucleated bone marrow cells and CFU-GM, improving the marrow morphology. The transplanted mMSC could be detected in the recipients 42 days after transplantation. Conclusion: Solo-injection of mMSCs can enhance hematopoietic reconstitution in mice with critical irradiation injury. The period of imbedding is related to the dose of infusion.
