RP-HPLC in determination of plasma nonoxynol-9 level in New Zealand rabbit and its application in pharmacokinetic study
10.3724/SP.J.1008.2009.00549
- Author:
Cut LI-JUN
1
Author Information
1. Department of Pharmaceutical Analysis, Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Nonoxynol-9;
Pharmacokinetics;
Plasma concentration;
RP-HPLC
- From:
Academic Journal of Second Military Medical University
2010;30(5):549-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a RP-HPLC method for determining the plasma concentration of nonoxynol-9 and to apply the method in studying the pharmacokinetics of nonoxynol-9 in healthy female rabbits. Methods: A single dose of nonoxynol-9 (64 mg to each animal) was given to 6 healthy female rabbits (weighing 2. 0-2. 2 kg) via vagina. The plasma samples were collected and the concentrations of nonoxynol-9 were determined by RP-1-IPLC method using DiamonsilTM ODS column (200 mm × 4.6 mm,5 μm) and mobile phase of methanol-water (95 : 5) at a flow rate of 1. 0 ml/min. The excitation and emission wavelengths were 226 nm and 300 nm, respectively. The column temperature was 30°C. The pharmacokinetic parameters were calculated. Results: The calibration curve of nonoxynol-9 showed good linearity over the range of 0. 05-5. 00 μg/ml; both the intra- and inter-day precisions were lower than 10%. The recovery rates at three concentrations (0. 10,0. 50, and 2. 00 μg/ml) were (101. 67 ± 8. 12) % ,(100. 84±5. 42) % ,and (100. 29±3. 81) % ,respectively. The main pharmacokinetic parameters of nonoxynol-9 were as following: t1/2 (3. 75 ± 0. 87) h, t max (3. 2 ± 0. 4) h,Cmax (2. 75 ± 0. 32) μg/ml, AUC0-12 (12.72±2.14) μg. h ml-1 ,AUC0-∞(15. 05± 2. 84)ug . h ml-1 ,and MRT (6. 85± 1. 13) h. Conclusion: The established method is convenient, sensitive and specific, and can be used for determination of plasma nonoxynol-9 concentration and for its pharmacokinetic studies in rabbits.
