Establishment and characterization of a human hepatocellular carcinoma cell line CSQT-1 derived from portal vein tumor thrombus
10.3724/SP.J.1008.2009.00001
- Author:
Hua-Sheng HU
1
Author Information
1. Department of Oncological Comprehensive Treatment
- Publication Type:Journal Article
- Keywords:
Cancer stem cells;
Cell line;
Liver neoplasms;
Neoplasm circulating cells;
Portal vein
- From:
Academic Journal of Second Military Medical University
2010;30(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a human hepatocellular carcinoma cell line derived from portal vein tumor thrombus and study its biological characteristics. Methods: Specimens of liver cancer and its metastasis-portal vein tumor thrombi (PVTT) were freshly resected during operation. The tissues were primary cultured by collagenase I digestion method and proliferated. The cell line derived from PVTT grew well. The biological characteristics of the cells were studied by light microscopy, electron microscopy, chromosome analysis, and transplantation experiment. Results: The cell line derived from portal vein tumor thrombus, named CSQT-1, has been cultivated for one year and sub-cultured for more than 100 passages in vitro. The cells either grew as compact colonies (in clusters) or as a monolayered sheet, with a doubling time of 48 h, and exhibited a typical malignant epithelial morphology. The median range of chromosome number was 87-90. Tumor nodes were observed under the skin of nude mice after subcutaneous xenograft transplantation for a month. The established cell line stably expressed CD133. Conclusion: We have successfully established a human hepatocellular carcinoma cell line from PVTT.
