Spectratyping analysis of complementarity-determining region 3 of T-cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with pemphigus vulgaris
10.3724/SP.J.1008.2009.00291
- Author:
Qian WANG
1
Author Information
1. Department of Dermatology
- Publication Type:Journal Article
- Keywords:
Complementarity determining region 3;
Genetic polymorphism;
Pemphigus vulgaris;
T cell receptor
- From:
Academic Journal of Second Military Medical University
2010;30(3):291-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the changes of the complementarity-determining region 3 (CDR3) of T-cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with pemphigus vulgaris (PV), so as to understand the association of proliferated T cells with autoimmune diseases. Methods: Immunoscope spectratyping technique was used to analyze the distribution of TCR β chain CDR3 in 6 normal blood donors and the dominant CDR3 in the PBMCs in 6 PV patients. Results: The spectratypes of TCR BV subfamily CDR3 region was in a Gaussian distribution manner in all the 6 normal blood donors. The 6 PV patients, however, displayed abnormal proliferation, and oligoclonal expansion of the T cells was observed in TCR BV families with different CDR3 sequences. Conclusion: The abnormal spectratypes of TCR BV subfamily CDR3 region in the peripheral blood of PV patients might be related to the pathogenesis of PV.
