Isolation of endophytic fungi from Panax ginseng and their antifungal and antitumor activities in vitro
10.3724/SP.J.1008.2009.00699
- Author:
Li-Li XU
1
Author Information
1. Department of Pharmacognosy
- Publication Type:Journal Article
- Keywords:
Bioactivity;
Endophytic fungi;
Isolation;
Panax ginseng
- From:
Academic Journal of Second Military Medical University
2010;30(6):699-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate endophytic fungi from Panax ginseng and study their antifungal and antitumor activities. Methods: Endophytic fungi were isolated from 5-year-old garden ginseng and 15-year-old transplanted ginseng. The antifungal active endophytes were screened with Pyricularia oryzae P-2b model using mircodilution method, and the activities of endophytes against pathogenic fungi were tested in vitro. The antitumor activities of the endophytes were examined by MTT method in vitro. Results: Sixteen (33.3%) of the 48 endophytic fungi fully suppressed the activity of P. oryzae P-2b; 11 (22.9%) colonies showed satisfactory antifungal activities against Candida albicans, Cryptococcus neoformans, Trichophyton rubrum, and Aspergillus fumigatus; and 5 (10.4%) colonies showed satisfactory antitumor activities against tumor cell lines MKN45, LOVO, HepG2, and HL-60. Among the bioactive colonies, Yuan-25 showed best antifungal activity, with its MIC80 aganinst Trichophyton rubrum being 4 mg/L, which was similar to that of fluconazole. Yuan-27 showed the best antitumor activity, with its IC50 similar to that of doxorubicin. Conclusion: Isolated endophytic fungi of Panax ginseng has antifungal and antitumor activities and is worth further exploring.
