Relationship of chemokine IP-10 and its soluble CXCR3 in bile with acute graft rejection after liver transplantation
10.3724/SP.J.1008.2009.00655
- Author:
Liang XIAO
1
Author Information
1. Department of Liver Transplantation
- Publication Type:Journal Article
- Keywords:
Chemokine;
IP-10;
Liver transplantation;
Rejection;
sCXCR3
- From:
Academic Journal of Second Military Medical University
2010;30(6):655-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the dynamic changes of chemokine IP-10 and its soluble receptor CXCR3 in bile, and to explore its relationship with acute graft rejection (AR) after liver transplantation. Methods: A total of 28 patients who underwent orthotopic liver transplantation in our hospital between October 2007 and March 2008 were included in the present study. They were divided into 2 groups according to the presence of AR: AR(n = 8) and NAR(n = 20). Another 10 patients who underwent endoscopic nasobiliary drainage (ENBD) for cholelithiasis in our hospital served as controls. The levels of chemokine IP-10 and sCXCR3 in the bile were determined by ELISA assay on 1,3,5,and 7 days after transplantation in all the patients and on 1,3,and 7 days after the anti-rejection therapy with glucocorticoid in patients of AR group; the relationship between their levels and the rejection active index (RAI) obtained by Banff criteria were evaluated; and its diagnostic value for AR was assessed. Results: The levels of IP-10 and sCXCR3 in bile gradually increased after liver transplantation and reached the peak on the 5th day after transplantation, and starting from day 5 after transplantation, their levels were significantly higher in the AR group than in the NAR group and ENBD group (P<0.05). Besides, the levels of IP-10 and sCXCR3 in bile were significantly decreased after treatment with glucocorticoid (P < 0.05). On the day of AR diagnosis, the the levels of IP-10 and sCXCR3 in bile were significantly correlated with RAI (P < 0.05). On the 5th day after transplantation, the diagnostic sensitivity and specificity of IP-10 level for AR were 87.5% and 100%, respectively (cut-off point = 964.45 pg/ml); on the 7th day after transplantation, the diagnostic sensitivity and specificity of sCXCR3 were 87.5% and 80%, respectively (cut-off point=819. 35 pg/ml). Conclusion: The levels of IP-10 and sCXCR3 in bile are closed related with early graft acute rejection, and their levels may serve as a non-invasive diagnostic indicator for AR and outcome of anti-rejection therapy.
