Long term exposure to salt stress induces antioxidative response in Candida albicans
10.3724/SP.J.1008.2011.0139
- Author:
Feng YANG
1
Author Information
1. Department of Physiology
- Publication Type:Journal Article
- Keywords:
Antioxidant enzymes;
Candida albicans;
Lipid peroxidation, ROS;
Oxidative stress;
Salt stress
- From:
Academic Journal of Second Military Medical University
2011;32(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of long term exposure to high concentration of Sodium Chloride (NaCl) on resistance to oxidative stress in Candida albicans (C. albicans). Methods: Two C. albicans strains were challenged with 1M NaCl for 22 consecutive days. Spot assay was performed to compare the resistance to Hydrogen Peroxide and Miconazole between wild type and derivative strains. The transcription level as well as the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) were examined. Physiological parameters such as lipid peroxidation in terms of malondialdehyde (MDA), and cellular reactive oxygen species (ROS) level were determined. Results: The derivative strains were more resistant to Hydrogen Peroxide (10 mM) and Miconazole (4μg/ml) as compared to the wild type strains. The transcriptions of antioxidant enzymes (SOD1, CAT1, TTR1) were increaed 2 to 5 times; The activities of the antioxidant enzymes were significantly higher in the derivative strains (P <0.05); Lipid peroxidation was significantly alleviated (P<0.01); And cellular ROS level was significantly reduced (P <0.001). Conclusion: Long term exposure to salt stress increases the resistance to oxidative stress in C. albicans.
