Ischemia-reperfusion injury induced overexpression of autophagy-related genes in cardiomyocytes
10.3724/SP. J.1008.2011.00776
- Author:
Jian XIAO
1
Author Information
1. Department of Cardiothoracic Surgery
- Publication Type:Journal Article
- Keywords:
Autophagy;
Myocardial reperfusion injury;
Myocardiocytes
- From:
Academic Journal of Second Military Medical University
2011;32(7):776-778
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ischemia-reperfusion (IR) injury on the expression of autophagy-related gene in cardiomyocytes. Methods: Male adult SD rats, weighing 250-300g, were randomly divided into 2 groups, namely, the control group, in which rats underwent thoracotomy without left anterior descending coronary (LAD) ligation, and IR group, in which the left anterior descending coronary arteries were ligated for 45 min, followed by reperfusion for 2 h. Beclin 1 and Atg5 mRNA expression levels were examined by RT-PCR in the myocardium, and the LC3 protein was determined by Western blotting analysis. Results: Beclin 1 and Atg5 mRNA expression levels in IR group increased to 2.45 and 1.6 times those of the control group, respectively (P<0.05). The expression of LC3-II protein was increased, which led to significant increase of LC3-II/LC3-I ratio in IR group (1.4 vs 0.2, P<0.01). Conclusion: Ischemia-reperfusion injury can induce expression of Atg family, promoting autophagy and causing cardiomyocyte damage.
