Lipopolysaccharide activates B cells and the underlying mechanisms
10.3734/SP.J.1008.2011.01231
- Author:
Li QIAN
1
Author Information
1. Department of Etiology and Immunology
- Publication Type:Journal Article
- Keywords:
Function;
Lipopolysaccharides;
B-lymphocytes;
Signal transduction
- From:
Academic Journal of Second Military Medical University
2011;32(11):1231-1234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of lipopolysaccharide (LPS) on B cell function and the related signal transduction pathways. Methods Freshly purified splenic B cells (CD19+B) of mice were isolated by magnetic activated cell sorting. After stimulation with LPS, the culture supernatants were collected for immunoglobulin isotyping by cytometric bead array (CBA) technology. Flow cytometry was used to analyze costimulatory molecule expression and culture supernatants were also collected for measusuring IL-6, IL-10, IL-12p70 and TNF by CBA technology. PD98059 (ERK inhibitor), SP600125 (JNK inhibitor), SB203580 (p38 MAPK inhibitor) and PDTC (NF-κB inhibitor) were used to study the signal pathways for the induced secretion of IL-6, IL-10and TNF in B cells. Results LPS induced IgG1-κ and IgM-κ production, up-regulated the expression of CD40, CD80, CD86 and MHC class II, and promoted IL-6, IL-10 and TNF secretion by B cells. Furthermore, JNK, p38 MAPK, and NF-κB pathways regulated the secretion of IL-6, IL-10 and TNF n B cells. Conclusion LPS can regulate B cell function by inducing Ig production, cytokine secretion, and expression of costimulatory molecules.
