High performance liquid chromatography in determination of matrine and sophoridine contents in Ganlibao capsule
10.3724/SP.J.1008.2012.01267
- Author:
Hui WANG
1
Author Information
1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Ganlibao capsule;
High pressure liquid chromatography;
Matrine;
Sophoridine
- From:
Academic Journal of Second Military Medical University
2012;33(11):1267-1269
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a high performance liquid chromatography approach for determining matrine and sophoridine contents in Ganlibao capsule. Methods: Agilent NH2 (4. 6 mm × 250 mm, 5μm) column was used with 1% phosphoric acid: ethanol: acetonitrile (V/V/V=10:22: 68) as the mobile phase. The flow rate was 0. 8 ml/min, and the temperature was at 25°C. The detection wavelength was set at 210 nm. Results: The calibration curve of matrine showed a good linearity in the range of 5.21-156.3 μg/ml (r=l) and that of sophoridine in the range of 5. 29-158. 7 μg/ml (r=0.9999). The equations of linear regression of matrine and sophoridine were Y = 9. 586 IX + 1. 003 8 and Y = 7. 604 3X - 5.9201,respectively. The recovery was 98. 73% (RSD=3.67%) for matrine and 100. 10% (RSD = 2.24%) for sophoridine. The matrine content of 15 batches of Ganlibao capsule was (30.13 ± 0. 19)-(44.70 ±0.17) mg/g and the sophoridine content was (21.60 ± 0.25)-(52.07 ± 0.11) mg/g. Conclusion: The present method is simple, accurate, and reproducible, and it can be used for the quality control of Ganlibao capsule.
