High performance liquid chromatography in determining ursolic acid and oleanolic acid contents in Qinggansanjie granules
10.3724/SP.J.1008.2012.01263
- Author:
Zhao LIANG
1
Author Information
1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
High pressure liquid chromatography;
Oeanolic acid;
Qinggansanjie granules;
Ursolic acid
- From:
Academic Journal of Second Military Medical University
2012;33(11):1263-1266
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the contents of ursolie acid and oleanolic acid in Qinggansanjie granules for quality control of the preparation. Methods: High-performance liquid chromatography with a diode-array detector (HPLC-DAD) was used for determination at the following conditions: The column was Chromsil-C18 (4. 6 mm× 250 mm, 5 μm), mobile phase was A: 0. 1% (V/V) formic acid solution, B: methanol, A = B = 12 = 88. The flow rate was 1. 0 ml/min, the temperature of column was 25°C, the detection wavelength was set at 210 nm, the injection volume was 50 μl, and the running time was 25 min. Results: Ursolic acid and oleanolic acid could be separated on baseline, with the linear range being 6. 1-97. 6 μg/ml for ursolic acid and 6. 4-102 μg/ml for oleanolic acid. The results of intra-day and inter-day precisions were both within 2% (n = 3), and the average recovery was 95. 4% (RSDl.54%, n = 6) and 99. 2% (RSDl.97%, n=6). Conclusion: The present method is simple, rapid, accurate, and with satisfactory repeatability; it can be used for quality control of ursolic acid and oleanolic acid in Qinggansanjie granules.
