Change of Dock1 protein expression in non-infarcted myocardium in rats
10.3724/SP.J.1008.2012.00540
- Author:
Xiao-Ian LIU
1
Author Information
1. Division of Cardiology, Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Dockl protein;
Guanine nucleotide exchange factor;
Myocardial infarction;
Myocardium
- From:
Academic Journal of Second Military Medical University
2012;33(5):540-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of Dockl protein expression in non-infarcted myocardium in rats. Methods Experimental myocardial infarction models were established in 22 SD rats (MI group) and the other 20 rats received sham-operation (Sham group). Eight rats were sacrificed 24 h after operation in each group and another 8 were sacrificed at 12 weeks after operation; the cardiac specimens were collected. Dockl protein expression was measured by Western blotting analysis in the non-infarcted myocardium of MI group and sham group. Results Dockl protein expression was not significantly different in the non-infarcted myocardium between MI group (0. 13 ± 0. 03) and Sham group (0. 10 ± 0. 04) at 24 h after operation (P>0. 05); 12 weeks after operation, the expression in non-infarcted myocardium of MI group (0. 17 ± 0. 04) was significantly higher than that of Sham group (0. 11 ± 0. 05, P<0. 05). Dockl protein expression in non-infarcted myocardium was significantly increased in MI group at 12 weeks after operation compared with those in MI and Sham groups at 24 h after operation (P<0. 05). Conclusion There is Dockl protein expression in rat's myocardium, and the expression is significantly increased in the non-infarcted myocardium at 12 weeks after infarction.
