Effects of sophoridine on cardiac function and myocardial ultrastructure of rats with myocardial infarction
10.3724/SP.J.1008.2012.0047
- Author:
Hon LU
1
Author Information
1. Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Heart function;
Myocardial infarction;
Sophoridine;
Ultrastructure
- From:
Academic Journal of Second Military Medical University
2012;33(5):470-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of sophoridine on cardiac function and myocardial ultrastructure of rats with myocardial infarction. Methods The SD rat model of myocardial infarction (MI) was obtained by permanent ligation of the left anterior descending coronary artery (LAD), and the model ratswere randomly divided into model group (rats with MI), lowdose group (5 mg/kg sophoridine), middle-dose group (10 mg/kg sophoridine), and high-dose group (20 mg/kg sophoridine); a sham operated group was also included. Each group had 10 rats. The elevated left ventricular end-diastolic pressure (LVEDP), ±dp/cdmax and heart mass/body mass (HM/BM) were determined after experiment, and the changes of myocardial ultrastructure were observed by transmission electron microscope. Results Compared with the sham operated group, ±dp/cdmax in MI group was significantly decreased, and LVEDP and HM/BM were remarkably increased (all P<0. 01). Compared with MI group, ±dp/cdm in the 3 sophoridine groupswere significantly increased, and LVEDP and HM/BM were significantly decreased (P<0. 01, P<0. 05), except for-dp/cdmax in the low-dose sophoridine group. Transmission electron microscope foundthat the myofilaments of myocardial cells in the sham operated group were well arranged, with normal mitochondria. The myofilaments were dissolved in MI rats, with swollen mitochondria and blurred cristae, showing distinct pathological changes. Therewas a slight improvement in myofilament dissolution in the low-dose sophoridine group, and the myofilament dissolution disappeared in the middle and high-dose group, with well arranged myofilaments. Conclusion Sophoridine can improve the cardiac function of MI rats, and has protective effects on ultrastructure of myocardial cells.
