A new method to create experimental model of noninvasive fungal sinusitis in rabbits
10.3724/3724/SP.SP.J.1008.2012.00016
- Author:
Jian WANG
1
Author Information
1. Department of Otorhinolaryngology
- Publication Type:Journal Article
- Keywords:
Animal models;
Fungi;
Noninvasive;
Sinusitis
- From:
Academic Journal of Second Military Medical University
2012;33(1):16-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a new method for creating experimental model of noninvasive fungal sinusitis in rabbits. Methods twenty five New Zealand white rabbits (50 sides) were divided into seven groups: control group (n = 6 sides), sham-operation group (n = 6 sides), mucosal-scratched group (n = 6 sides), ostia-blocked group(n = 6 sides), Aspergillus fumigatus inoculated group(n = 8 sides), mucosal-scratched plus Aspergillus fumigatus inoculated group (n = 9 sides), and ostia-blocked plus Aspergillus fumigatus inoculated group(n = 9 sides). The experiment was divided according to whether the maxillary sinus mucosa was wounded or not and whether the natural ostia were blocked or not On days 14 and 28 after Aspergillus fumigatus inoculation, histopathologic and mycological examinations of both maxillary cavities and mucous membrane were performed to con formative establishment of the model. Results Aspergillus fumigatus plus ostia obstruction group had a positive rate of 88. 9% (8/9 sides) cm day 14 and 77. 8% (7/9 sides) cm day 28 in fugal culture. Aspergillus fumigatus plias mucosa wound yielded a positive rate of 44. 4% (4/9 sides) on day 14 and 22. 2% (2/9 sides) on day 28. Conclusion Aspergillus fumigates inoculation combined with an blocked ostium can be used to establish experimental model of noninvasive maxillary fungal sinusitis.
