Experimental research on stabilizing microtubules to decrease myocardial injury
10.3724/SP.J.1008.2013.00167
- Author:
Jian FENG
1
Author Information
1. Department of Cardiology, Wuxi People's Hospital Affiliated to Nanjing Medical University
- Publication Type:Journal Article
- Keywords:
Ischaemic ventricular arrhythmia;
Microtubules;
Myocardial infarction;
Reperfusion injury;
Taxol
- From:
Academic Journal of Second Military Medical University
2013;34(2):167-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study whether stabilizing microtubules can decrease myocardial ischaemic-reperfusion injury. Methods: (1) The isolated rat hearts were divided into four groups (n = 15): control group, ischemic group, ischemic+0. 1 μmol/L Taxol group, and ischemic+1 μmol/L Taxol group. All the groups were given a 15-min equilibration and then followed by different treatments: control group, 50 min normoxic superfusion; ischemia group, 20 min normoxic superfusion plus 30 min ischemia; and Taxol groups, 20 min normoxic superfusion plus 30 min ischemia, plus 0. 1 or 1 μmol/L Taxol throughout 50-min superfusion period. Arrthymias scores were assessed and compared between different groups; the structure of the microtubules was observed and its breakage score was obtained. (2) The isolated rat hearts were divided into 3 groups (n = 8): normal control group, ischemic/reperfusion (I/R) group, and 1 μmol/L Taxol group. The I/R group was Langendorff-perfused; the left anterior descending branch was ligated for 30 min and reperfused for 120 min. The Taxol group received 1 μmol/L Taxol and other treatments were similar to the I/R group. Evans blue perfusion was used to observe the infarct size of each group. Results: Stabilizing microtubules with Taxol (0. 1 μmol/L or 1 μmol/L) reduced ischaemic ventricular arrhythmia in a dose-dependent fashion (P < 0. 05); it also significantly reduced arrhythmia scores and the incidence of ventricular tachycardia (P < 0. 05). Taxol at 0. 1 μmol/L greatly decreased microtubule breakage score, and at 1 μmol/L significantly reduced the infarct size compared with the control group (P < 0. 05). Conclusion: Stabilizing microtubules can reduce myocardial ischaemic-reperfusion injury.
