Inhibitory effect of botulinum toxin type a against exogenous ACh- and substance P-induced contraction of rat lower esophageal sphincter in vitro
10.3724/SP.J.1008.2013.00164
- Author:
Chao-Yan LI
1
Author Information
1. School of Basic Medical Sciences, Luohe Medical College
- Publication Type:Journal Article
- Keywords:
Acetylcholine;
Botulinum toxin type A;
Lower esophageal sphincter;
Substance P
- From:
Academic Journal of Second Military Medical University
2013;34(2):164-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study whether botulinum toxin type A (BTX-A) can inhibit the spontaneous and acetylcholine (ACh)- or substance P (SP)-induced contraction of rat lower esophageal sphincter in vitro, and to discuss the related mechanism. Methods: The lower esophagus muscle strips were taken from Sprague-Dawley rats and were randomly divided into control group, BTX-A group, ACh group, ACh+BTX-A group, ACh+Atropine group, SP group, SP+APTL-SP group and SP+BTX-A group. The contractile graph of the muscle strips was recorded by physiological experimental system Biolap-420E. Results: BTX-A significantly decreased the spontaneous contractile tension and amplitude in the lower esophageal sphincter(P<0. 05). ACh significantly enhanced the contractile tension and amplitude in the lower esophageal sphincter (P<0. 01), which could be significantly inhibited by both BTX-A and Atropine (P<0. 01). SP significantly enhanced the contractile tension in the lower esophageal sphincter (P<0. 01), which could be significantly inhibited by both BTX-A and APTL-SP (P<0. 01). Conclusion: ACh and SP can enhance the spontaneous contractility of lower esophageal sphincter. BTX-A can inhibit ACh- and SP-induced enhancement of lower esophageal sphincter contraction.
