Simultaneous determination of six marker components in Sini decoction by high performance liquid chromatography
10.3724/SP.J.1008.2013.00682
- Author:
Min LIU
1
Author Information
1. The Institute of Quality Control of Medical Material and Equipment, The Jiont Logistic Department, PLA Nanjing Miliary Area Command
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2013;34(6):682-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective To simultaneously determine the contents of six marker components, including benzoylmesaconine, benzoylaconine, benzoylhyaconine, isoliquiritigenin, glycyrrhizic acid, and 6-gingerol, in Sini decoction by high performance liquid chromatography (HPLC). Methods The HPLC condition was as follows:column: Waters Terra C18 (3.0mm X100mm,.5jLtm); thedetective wavelength was set at 370 nm for isoliquiritigenin and 235 nm for the other five components. Mobile phase A was 95% acetonitrile+5% H2O (5 mmol/L ammonium acetate), B was 0.1% formic acid aqueous solution (5 mmol/L ammonium acetate), with gradient elution, the gradient of A phase 25%-35% (0-5 min), 35%-50% (5-15 min), 50%-85% (15-20 min); flow speed: 0. 5 mL/min temperature of column 25C; injection volume: 5 iL. Sini decoction was obtained by using the traditional decoction preparation method. Results Benzoylmesaconine, benzoylaconine, benzoylhyaconine, isoliquiritigenin, glycyrrhizic acid and 6-gingerol were separated at baseline within15 min, showing good linearity (r>0. 999 0) between (5. 600-112. 0) jig/mL, (6. 560-131. 2) μig/mL, (6. 130-122. 6) μig/mL, (4. 590-91. 8) μig/mL, (31. 00-620. 0) fig/mL, and (4. 920-98. 4) μig/mL, respectively. The results of intra-day and inter-day precisions were at normal range (RSD<5%), with the recovery rates (n = 6) being 101. 07% (RSD= 1. 3 %), 98.72% (RSD= 1. 1%), 101.57% (RSD= 1. 8%), 101.71% (RSD=3. 6%), 102.12% (RSD=2. 3%), and 99.58% (RSD=3. 8%), respectively. Conclusion The present method is rapid, simple, and accurate and it can be used to determine the above 6 components in Sini decoction.
