Preparation of CDllc modified immunoliposome and validation of its targeting to dendritcc cells
10.3724/SP.J.1008.2013.00628
- Author:
Zhan WAN
1
Author Information
1. School of BasicMedical Sciences, Hebt North University
- Publication Type:Journal Article
- Keywords:
CD11c antigens;
Dendritic cells;
Liposomes;
Target
- From:
Academic Journal of Second Military Medical University
2013;34(6):628-632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and characterize liposome with mAb(CD11c-imliposomes) and to validate its targeting to dendritic cells (DCs) in vitro. Methods Liposomes were prepared by film dispersion method, and CD11c mAbwas collected to the surface of liposome by chemical cross-linking method. The conjugation efficiency ofCD11c mAb to the liposome was determined by HPLC; the morphology of CD11c-imliposomes was observed under transmission electron microscope; the particle size and Zata potential were determined by dynamic light-scattering; the activity of CD11c mAb was examined by flow cytometry; and its specific targeting to DCs was visualized by laser scanning confocll microscopy. Results The CD11c-imliposomes were successfully prepared and the conjugation efficiency was (77. 6±3. 2)%, with the particle size being (151. 2±1. 6) nm and the Zeta potential being (- 32. 0±1. 8) mV. Under microscope, the CDllc-imliposomes were mostly round in shape or elliptical, with smooth surface and with no adhesion. The activity of CD11c-imliposomes was still satisfactory and demonstrated great targeting to DCs in vitrr. Conclusion The CD11c-imliposomes prepared by us has targeting ability to DCs in vitro.
