Effect of recombinant human endostatin on VEGF and TIMP-1 expression in hypetrophic scar of rabbit ears
10.3724/SP.J1008.2013.0054
- Author:
Peng WANG
1
Author Information
1. Department of Orthopedics, People' Hospital of Dazu District
- Publication Type:Journal Article
- Keywords:
Cicatrix;
Recombinant human endostatin;
Tissue inhibitor of metalloproteinase-1;
Vascular endothelial growth factors
- From:
Academic Journal of Second Military Medical University
2013;34(5):549-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of recombinant human endostatin on the expression of vascular endothelial growth factor (VEGF) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in hypertrophic scar (HS) of rabbit ears. Methods A total of 16 New Zealand rabbits were used to establish ear HS models. The model rabbits were evenly randomized into two groups: experimental group and control group. Rabbits in the experimental group were locally injected with recombinant human endostatin (0. 1 mL, 5 mg/mL, every other day for five times) and those in the control group were injected with normal saline (0. 1 mL, every other day for five times). The HS specimens were harvested 30 days after intervention and were grossly observed; RT-PCR was used to test the expression of VEGF and TIMP-1 mRN, and TIMP-1 protein was examined by immunohistochemistry method. Results The mRN A expression levels of VEGF and TIMP-1 in experimental group were significantly lower than that in control group (VEGF: 0. 279 0 + 0. 030 7 vs 0. 657 0 ± 0. 161 1, P< 0. 05 TIMP-1: 0.244 4+ 0. 057 4 vs 0. 730 2 + 0. 103 8, P<0. 05). The protein expression level of TIMP-1 in experimental group was significantly lower than those in control group (P<0. 05). Conclusion Recombinant human endostatin can inhibit HS of rabbit ear, which might be through suppressing the expression of VEGF and TIMP-1.
