LC-MS/MS in determination of Bletilla striata glucomannan in Blettila striata hemostatic sponge
10.3724/ISP.J.1008.2014.01401
- Author:
Wei WANG
1
Author Information
1. Department of Pharmacy, No. 455 Hospital of PLA
- Publication Type:Journal Article
- Keywords:
Bletilla striata glucomannan;
Blettila;
Liquid chromatography-mass spectrometry
- From:
Academic Journal of Second Military Medical University
2014;35(12):1401-1403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a LC-MSIMS system for the determination of Bletilla striata glucomannan in Bletilla striata hemostatic sponge. Methods The Waters X Bridge™ Amide(3. 5 [J-ill, 2. 1 mm X 100 mm) column was used at 35 'C; acetonitrile and 5 mmol/L ammonium acetate (containing 0.1% formic acid) (60: 40, VIV) were used as the mobile phase at a flow rate of 0. 3 mLimin, with the injection volume being 10 [J-L. Results The linear range for D-mannose was 50. 0-5 000. 0 nglmL (r=O. 999 5). The results of intra-day and inter-day precisions, limit of detection and limit of quantitation were all within the normal ranges. The recovery rate (n=5) was 98. 4%, RSD= 1. 03%. Conclusion The method in this study is simple, selective and sensitive; it can be used for the quantitative determination and quality control of Bletilla striata glucomannan.
