Expression of CD133 and CDLO5 on cells in peripheral blood of patients with primary hepatocellular carcinoma
10.3724/SP.J.1008.2014.01382
- Author:
Yong XU
1
Author Information
1. Department of Minimally Invasive Surgery, The Third Affiliated Hospital of Anhui Medical University
- Publication Type:Journal Article
- Keywords:
CD105;
CD133;
Flow cytometry;
Primary hepatic carcinoma;
Prognosis
- From:
Academic Journal of Second Military Medical University
2014;35(12):1382-1386
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of CD133+ and CD105+ cells in the peripheral blood with the clinical prognosis of patients with primary hepatocellular carcinoma (HCC). Methods The proportions of CD45- CD133+ CD105+ cells were determined in 80 primary HCC patients and 20 healthy subjects by flow cytometry, and the correlation of the proportion with age, gender, clinical stage, histological differentiation, lymph node metastasis, and surgical outcomes were analyzed. The survival rate was analyzed by Kaplan-Meier curves, and multivariate analysis was performed using Cox proportional hazards model. Results The proportion of CD45- CD133+ CD105+ cells in the peripheral blood of primary HCC patients was significantly higher compared with those in the postoperative patients and healthy controls (P= 0. 003, P= 0. 000). The proportion of preoperative CD45- CD133+ CD105+ cells in the postoperative recurrent group was significantly higher than those in the non-recurrent group (P=O. 015). The proportion of CD45- CD133+ CD105+ cells in the peripheral blood of primary HCC patients was significantly associated with the clinical stage, degrees of differentiation, and presence/absence of lymph node metastasis CP 0. 5% compared with 0. 5% CP 1. 1% was significantly lower than that with CD45- CD133+ cell proportion 1. 1% CP 36% was significantly lower than those with CD45- CD105+ proportion36% CP
