Impact of CYP3A5 and ABCBL polymorphisms on drug switching from cyclosporine to tacrolimus for individualized treatment of renal transplant recipients
10.3724/SP.J.1008.2014.01320
- Author:
Xue-Bin WANG
1
Author Information
1. Department of Pharmacy, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Cytochrome P-450 CYP3A5;
Kidney transplantation;
P-glycoprotein;
Single nucleotide polymorphism;
Tacrolimus
- From:
Academic Journal of Second Military Medical University
2014;35(12):1320-1326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of CYP3A5 and ABCB1 polymorphisms on the initial individualized treatment with tacrolimus (FK506) in renal transplant recipients during switching from cyclosporine (CsA) to FK506. Methods Polymerase chain reaction and restriction fragment length polymorphism method (PCR-RFLP) was employed to investigate CYP3A5 (A6989G) and ABCB1 (exon12 [C1236T], exon21G[A] 2677T and exon26 [C3435T]) genotype data. The initial trough concentration/ dose (Co/D) values were compared among different CYP3A5 genotypes in renal transplant recipients switching from CsA to FK506 using one-way ANOVA. In addition, the initial C0 /D values were also compared among different ABCB1 (exon12[C1236T], exon21G[A]2677T and exon26[C3435T]) genotypes and their haplotypes using one-way ANOVA. Results The FK506 C0 /D values were significantly different between different CYP3A5 genotypes (AA, AG and GG) at the 7th, 14th, 21th and 28th day of conversion from CsA to FK506 in renal transplant recipients CP
