SDS-NaOH solution for rapid discrimination of gram-positive and gram-negative bacteria in urine
10.3724/SP.J.1008.2014.00907
- Author:
Hu LI
1
Author Information
1. Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Bacteria;
Gram staining;
SDS-NaOH solution;
Urinary tract infections;
Urine
- From:
Academic Journal of Second Military Medical University
2014;35(8):907-909
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficiency of SDS-NaOH solution for discriminating Gram-positive and Gram-negative bacteria in urine. Methods Seventy-nine urine samples were treated with SDS-NaOH solution for 5 minutes. The bacterium counts were determined by UF-1000i urine analyzer before and after treatment, and the bacteria ratio (BR) was calculated. Receiver operation characteristics (ROC) curve analysis was used to assess the discrimination efficiency of B.R. Results BR in Gram-negative bacteria contained urine was significantly lower than that in Gram-positive one (P=0.01). The area under curve (AUC) of BR for discriminating Gram-positive and Gram-negative bacteria was 0.70 (95% confidence interval:0.57-0.82). At a cut-off value of 0.05, the discrimination sensitivity and specificity of BR for Gram-positive bacteria were 0.83 (95% confidence interval:0.63-0.95) and 0.46 (95% confidence interval:0.32-0.59), respectively. Conclusion Treatment with SDS-NaOH solution can help to discriminate Gram-positive and Gram-negative bacteria in urine, and the method is rapid and automatic.
