Breeding and genotype identification of NLRP3 gene knock-out mice
10.3724/SP.J.1008.2014.00555
- Author:
Shi-Jie FENG
1
Author Information
1. Department of Health Toxicology, College of Tropical Medicine and Public Health, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Heterozygote;
Homozygote;
Inflammasome;
Knockoutmice;
Mice;
NLRP3
- From:
Academic Journal of Second Military Medical University
2014;35(5):555-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To breed and identify NLRP3 gene knock-out mice. Methods: The NLRP3 gene knock-out heterozygote mice were bred alone and copulated. The offsprings were to have three genotypes: wild genotype, heterozygote genotype and homozygote genotype. Genomic DNA was obtained from each pups and were subjected to PCR and T7 endonuclease 1 to identify the genotype. The homozygote mice were mated with the opposite sex heterozygote mice to obtain more homozygote pups. Results: Breeding and reproducing were both successful, and we obtained heterozygote genotype and homozygote genotype mice with NLRP3 gene knock-out. Conclusion: Correct methods of breeding, reproducing and identifying can effectively obtain NLRP3 gene knock-out mice from heterozygote mice.
