Protective effect of VEGF receptor 2 on cone photoreceptors under hypoxia condition
10.3724/SP.J.1008.2014.00482
- Author:
Ke SHI
1
Author Information
1. Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University
- Publication Type:Journal Article
- Keywords:
Anoxia;
Cones (retina);
Electroretinography;
Vascular endothelial growth factor receptor-2
- From:
Academic Journal of Second Military Medical University
2014;35(5):482-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of VEGF receptor 2 (VEGFR2) on retinal cone photoreceptors under hypoxia condition. Methods: Eigh--week-old cone-specific VEGFR2-knockout (KO) mice and wild-type (WT) C57BL/6 mice received intravenous injection of 8 mmol/L cobalt chloride in the right eyes for hypoxia condition and PBS in the left eyes for control, with 6 mice in each group. On the next day electroretinogram (ERG) was conducted to evaluate the photoreceptor function, and immunofluorescence colocalization was used to determine the density and morphology of cone photoreceptors. Results: In KO and WT groups, hypoxia eyes had significantly decreased a-wave and b-wave amplitudes in scotopic ERG compared with PBS control eyes (P<0. 01), but with no significant ditference between hypoxia eyes of KO and WT groups (P>0. 05). In both KO and WT groups, the hypoxia eyes also had significantly decreased a-wave and b-wave amplitude in photopic ERG compared with PBS control eyes (P<0. 01), and the decrease in KO mice was more significant than that in WT group (P<0. 05, P<0. 01), indicating the decrease of cone function in KO animal. Morphology examination showed noticeable changes under hypoxia condition in both KO and WT groups, showing decreased density, shortened appearance, and disordered arrangement, with the changes in KO group being more noticeable. Conclusion: VEGFR2 has a protective effect on cone photoreceptors under hypoxia condition.
