Effects of U75302, blocker of leukotriene B4 receptor 1, on cellular immunity and inflammation in septic mice
10.3724/SP.J.1008.2014.00246
- Author:
Xiu-Juan LI
1
Author Information
1. Department of Anesthesiology, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Cellular immunity;
Inflammation;
Leukotriene antagonists;
Leukotriene B4 receptor 1;
Sepsis
- From:
Academic Journal of Second Military Medical University
2014;35(3):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of U75302, blocker of leukotriene B4 receptor 1, on immune function in septic mice, so as to explore the implication of blocking leukotriene B4 receptor 1 for treatment of sepsis. Methods Experimental sepsis model was induced by cecal ligation and puncture (CLP). A total of 18 male C57BL/6 mice were randomly divided into sham group (n=6), CLP group (n=6) and CLP + U75302 intraperitoneal injection group (n = 6). The peripheral blood cytokine tumor necrosis factor- α (TNF-α) and interleukin-10 (IL-10) levels, peritoneal lavage fluid Gr-1+ cell count and thymus T lymphocytes apoptosiswere detected in mice of three groups 24 h after surgery. Results Compared with the CLP group, CLP + U75302 intraperitoneal injection group had significantly reduced blood TNF-α level (by 43%, P< 0. 05), significantly increased IL-10 levll (by 88%, P<0. 05), signficantly decreased Gr-1+ cell counts in peritoneal lavage fluid (P<0. 05), and significantly decreased apoptosis of thymus T lymphocytes (P<0. 01). Conclusion Blocking leukotriene B4 receptor 1 with U75302 may decrease peripheral TNF-α level, increase IL-10 level, and improve cellular immunity, which may be involved in inflammation of sepsis.
