Effects of basic fibroblast growth factor on chondrocyte phenotype and dedifferentiation in porous tantalum-chondrocyte composites in vitro
10.3724/SP.J.1008.2015.00248
- Author:
Hui ZHANG
1
Author Information
1. Graduate School, Southern Medical University
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Cell dedifferentiation;
Chondrocytes;
Domestic porous tantalum;
Phenotype
- From:
Academic Journal of Second Military Medical University
2015;36(3):248-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of basic fibroblast growth factor (bFGF) of different concentrations on phenotypes and dedifferentiation of rabbit chondrocytes in porous tantalum-chondrocyte composites in vitro, so as to provide theoretic basis for cartilage defect repair. Methods The articular chondrocytes from 3-week-old rabbit were cultured and identified by type IT collagen immunocytochemistry and Safranin 0 staining. The 3rd generation chondrocytes were implanted in the porous tantalum and was treated with bFGF of various concentrations. The bFGF-chondrocyte-porous tantalum composites (bFGF compostes) were then divided into 5 groups: group A (1 ng/mL bFGF composites), group B (10 ng/mL bFGF composites), group C (50 ng/mL bFGF composites), group D (chondrocyte-porous tantalum), and group E (pure chondrocyte). The proliferation of chondrocytes was measured by MTT and the cell morphology and growth were observed by scanning electron microscopy (SEM). Phenotypes and dedifferentiation (type I, II, IX, and X collagen) of the chondrocytes were detected by immunocytochemical method. Type II and X collagen mRNA was tested by real-time PCR. Results Type II collagen immunocytochemistry and Safranin 0 staining were positive, confirming that the cultured cells were chondrocytes. MTT results showed that chondrocyte proliferation in groups A, B, C, and D were significantly greater than that in group E CP
