Effect of rapid eye movement sleep deprivation on expression of neuropeptide s in dorsomedial nucleus of rat hypothalamus

Author: Fei ZHAO ¹
Author Information

1. Department of Neurology, Changzheng Hospital, Second Military Medical University
Publication Type:Journal Article
Keywords: Dorsomedial hypothalamic nucleus; Neuropeptide S; REM sleep parasomnias; Sleep deprivation
From: Academic Journal of Second Military Medical University 2015;36(3):320-323
CountryChina
Language:Chinese
Abstract: Objective To observe the changes of the neuropeptide S (NPS) expression in dorsomedial nucleus of rat hypothalamus after rapid eye movement (REM) sleep deprivation. Methods Totally 24 adult male rats were evenly randomized into 3 groups: normal control group (CC), tank control group (TC) and REM sleep deprivation group (SD). Modified multiple platform method (MMPM) was used to establish REM sleep deprivation model in rats. Immunohistochemistry and hybridization in situ were applied to examine the expression of NPS protein and mRNA in the rat hypothalamus. Results There was no significant differences in the expression of NPS protein or mRNA in rats hypothalamus between CC group and TC group. After REM sleep deprivation, NPS protein and mRNA positive cells in rats hypothalamus in SD group were (27. 86 ± 2. 47) and (25. 75±2. 12), respectively, which were significantly more than those in CC group and TC group (16. 75 ± 2. 12 and 19. 63 ± 1. 85 in CC group, 18. 60±1.60 and 18. 50±1.69 in TC group; P