Inhibitory effects of hydrogen-rich water against tumor growth in tumor-bearing mice with colorectal cancer and the molecular mechanisms
10.16781/j.0258-879x.2016.09.1082
- Author:
Yao ZHANG
1
Author Information
1. College of Agricultural and Biological Sciences, Shanghai Jiaoiong University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Colorectal neoplasms;
Hydrogen;
Interferon-γ-related genes;
Oxidative stress
- From:
Academic Journal of Second Military Medical University
2016;37(9):1082-1087
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigaie the inhibitory effects of hydrogen-rich water ([H]H2O) against tumor growih in mice with colorectal cancer and the related molecular mechanisms. Methods Mice were vaccinated with SW480 cells suspension (4 × 106 cells/mice) 10 establish mouse model of colon cancer. The mice in [H]H2O intervention group were freely allowed to drink ([H]H2O)(0. 8 mg/kg), which was refreshed three limes daily, and die mice in control group were given lighi water. The lumor weight and volume were measured 28 d after bearing lumor lo assess tumor growth, the expression of Ki-67 protein was examined by immunohisiochemical method- the apoptosis of lumor cells was observed by TUNEL. and the levels of oxidized glutathione (GSSG) and glutathione (GSH) were analyzed using colorimeiric method Micro array was used to screen the differentially expressed genes between control group and ([H]H2O) intervention group, and the levels of those genes were verified with qRT-FCR. Results Compared with the control group. ([H]H2O) intervention significantly inhibited the tumor growth, reduced tumor weight and volume (P<0. 01, P<0. 05); moreover, it also reduced the expression of Ki-67 protein (P<0. 05) and promoted tumor cell apoptosis (P<0. 05). In addition, the ratio of GSSG GSH was significantly increased in [H]H2O(> intervention group compared with that in the control group (P<0. 05). ([H]H2O) intervention could also regulate the expression of interferon γ-related genes. Conclusion ([H]H2O) may exert its antitumor activity via inhibiting proliferation, inducing apoptosis of colon cancer cells, and regulating the intracellular redox state and the expression of interferon γ-related genes.
