Experimental research of isoflavone-loaded mesoporous glass cement scaffold materials for rabbit-femoral bone defect repair
10.16781/j.0258-879x.2016.02.0184
- Author:
Yong WANG
1
Author Information
1. Department of Trauma Orthopaedics, Wenzhou Traditional Chinese and Western Medicine Hospital
- Publication Type:Journal Article
- Keywords:
Bone repair;
Bone substitutes;
Caicium sulfate;
Isotlavones;
Mesoporous magnesium caicium silicate
- From:
Academic Journal of Second Military Medical University
2016;37(2):184-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficacy of isoflavone (IS)-loaded mesoporous glass cement scaffold materials in repairing the rabbit femoral bone defect. Methods Various composite glass cements absorbing plant hormones (soy isoflavone) were fabricated as follows: calcium sulphate (CS), 20% mesoporous magnesium calcium silicate/calcium sulfate (20 m-MCS/ CS) , and 4:0% mesoporous magnesium calcium silicate/calcium sulfate (40 m-MCS/CS). The in vitro isoflavone release profiles of the above materials were evaluated. Sixty New Zealand rabbits were randomized into 4 groups with 15 in each group, and the bone defect models in the distal end of femur were created. Group A: implanted with CS; Group B: 20 m MCS/CS; Group C: 40 m-MCS/CS; and Group D: IS-loaded 40 m-MCS/CS (40 m-MCS/CS/IS). The rabbits were sacrificed at 4 weeks, 8 weeks and 12 weeks postoperatively and the bone samples were scanned with micro-CT. Then histology analysis was conducted under microscope after decalcification and Masson trichrome staining. Results Controlled release profiles of isoflavone were presented in both 20 m-MCS/CS/IS and 40 m-MCS/CS/IS groups, and a higher cumulative release rate was detected in 40 m-MCS/CS/IS group, which reached (41. 0 ± 1. 8)% on the 25th day; therefore 40 m-MCS/CS/IS was selected for further evaluation in vivo, and 40 m-MCS/CS, 20 m-MCS/CS and CS served as controls. Rabbits in Group A implanted with CS showed litte new bone formation 12 weeks after operation; those in Group B implanted vith 20 m-MCS/CS presented with better bone regeneration in the defect stte; and the bone defect sites in group C grafted with 40 m-MCS/CS were almost filled with neo-bone; however, the best results were obtained in group D which was implanted with 40 m-MCS/CS/IS, with the defects fully repaired by the bone trabecular. Conclusion Isotlavone-loaded mesoporous glass cement can effectively repair lacunar bone defects, and may serve as a promising new bone regeneration material.
