Study on the biological properties of strontium doped hydroxyapatite
10.16781/j.0258-879x.2016.02.0138
- Author:
Jian-Yong GAO
1
Author Information
1. Department of Stomatology, Changhai Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Bioactivity;
Biocompatibility;
Hydroxyapatites;
OSteoblasts;
Strontium
- From:
Academic Journal of Second Military Medical University
2016;37(2):138-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biocompatibility and biological activity of hydroxyapatite (HA) with different strontium (Sr) contents. Methods The Sr doped HA (Sr-HA) materials containing 0%, 1%, 5%. 10%, and 20% Sr were prepared and the concentrations of Ca2+1 and Srz2+ in the leaching liquor of them were determined in each group. Hemolysis tests were performed in vitro for the 5 materials. L929 fibroblasts were seeded and cultured with leaching liquor of materials for 2 d and 7 d; cell morphology was observed under microscope and the invitro cytotoxicity of the five materials was detected by MTT assay. The MG63 bone cells were seeded on the surface of the 5 materials; then cell adhesion was observed at 1, 4, 12 h of culture, and the proliferation of cells was observed on day 1, 4 and 7 of culture by MTT method; and alkaline phosphatase (ALP) activity of the osteoblasts was detected on day 1, 3, and 5. Results The highest Ca2+ concentration was found in the leaching liquor of 5% Sr group, and the lowest was found in the 1% Sr group; the highest Sr2+ concentration was found in the leaching liquor of 20% Sr group, and the lowest was found in 0% Sr group. The hemolytic rates of Sr-HA materials were lower than 5% in all the five Sr-HA groups. The morphology of L929 fibroblasts in the five Sr-HA groups was similar to that of the negative control group; and MTT assay showed that the overall cytotoxicity of the five Sr-HA groups was grade 0 or 1. After 4 and 12 h culture, the number of adhered MG63 osteoblasts in 5%, 10%, and 20% Sr groups were significantly more than those in 0% and 1% Sr Groups (P<0. 05, P<0. 01). On day 4 and 7 of culture, the proliferation rates 0f MG63 Osteoblasts in 5%, 10% and 20% Sr groups were significantly higher than those in 0% and 1% Sr groups (P<0. 05). The ALP activity 0f MG63 Osteoblasts in 5% and 10% Sr groups was significantly higher than that in 0% and 1 % Sr group on day 3 of cuuire (P< 0. 05) ; and that in 5% , 10% , and 20% Sr group was higher than that in 0% and 1 % Sr group 0n day 5 0f culture Conclusion Sr-HA material with different Sr contents has good biocompatibility; the combinati0n with certain concentration of Sr can effectively improve the biological activity of HA, with the best activity Seen when Sr rati0 ss 5% 0r 10%.
