Inhibitory effects of luteolin against proliferation of gastric cancer cell line MGC803 and the related mechanisms
- Author:
Xiang Qiang ZHANG
1
Author Information
1. Department of Physiology, Medical College, JinanUniversity
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Autophagy;
Cell cycle;
Luteolin;
Stomach neoplasms
- From:
Academic Journal of Second Military Medical University
2016;37(3):302-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of luteolin against proliferation of gastric cancer cell line MGC803 and the related mechanism. Methods The inhibitory effect of luteolin against MGC803 cell proliferation was evaluated by MTT assay and clone forming assay. The cell cycle changes were analyzed by the flow cytometry after staining propidium iodide (PI). Annexin VPI double staining was used to determine the apoptosis ratio of MGC803 cells. Bcl-2 family proteins. Caspase proteins. autophagy-associated and cell cycle-associated proteins were assessed by Western blotting analysis. Results MTT assay results showed that the IC50 of luteolin against MGC803 cells for 24 h. 48 h and 72 h treatment were 127. 37 µmol/L. 76. 12 µmol/L. and 16. 84 µmol/L. respectively. Luteolin treatment also inhibited cell colony formation of MGC803 cells. PI single staining flow cytometry found that cells were arrested in G2 phase with the increase of luteolin concentration. Western blotting results showed that, with the increase of luteolin concentration. CDK4, CDK6 and CyclinE2protein expression was decreased; CyclinDl, CyclinD3 and p-Weel protein kept unchanged; and CyclinA, CyclinB, Mytl and p- cdc2 (Tyrl5) protein expression was decreased. Flow cytometry Annexin V^FITC/PI revealed increase of early and late apoptosis with the increase of luteolin concentration. Western blotting analysis showed that Mcl-l, Bcl-xL, Caspase-3, Caspase- 8 and Caspase-9 protein expression was decreased with the increase of luteolin concentration, with slightly changed Be 1-2 expression and increased Bax expression, which led to decreased Bcl-2/Bax, Mcl-l/Bax and Bcl-xL/Bax ratios. At the same time, Caspase-3 and PARP also appeared in the strip cutting. The expression of P62 was decreased and expression of Beclin-l and LC3 " was increased. Conclusion Luteolin can arrest gastric cancer MGC803 cells at G2 phase, inducing autophagy and inhibiting cell growth. Luteolin can also induce apoptosis of MGC803 cells via the mitochondrial pathway.
