MicroRNA-484 regulates liver fibrosis course through targeting Fisl in hepatic stellate cells
10.16781/j.0258-879x.2017.09.1146
- Author:
Li-fen ZHANG
1
Author Information
1. Department of Pathology, Changzheng Hospital, Second Military Medical University
- Publication Type:Journal Article
- Keywords:
Activation;
Apoptosis;
Hepatic stellate cell;
Liver fibrosis;
MicroRNA-484;
Mitochondrial fission 1 protein
- From:
Academic Journal of Second Military Medical University
2017;38(9):1146-1151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of microRNA-484 (miR-484) on hepatic stellate cells (HSC's), the key cell in the occurrence and development of liver fibrosis, and to investigate the functions. Methods On the basis of our previous microarray analysis results, we transfected HSC-T6 cell lines with miR-484 inhibitor to intervene the expression of miR-484 in vitro. The expressions of mRNA and proteins related to liver fibrosis and apoptosis were detected by qPCR and Western blotting, respectively. The cell apoptosis with Annexin V-FITC/PI double staining was determined by flow cytometry. Results Compared with the control group, the mRNA and protein expression of miR 484-targeted gene Fisl and proapoptotic factor caspase-3 were both significantly up-regulated (P<0. 05), and apoptosis inhibitory factor Bcl-2 was significantly down-regulated (P
