Effect of LIM homeobox gene-8 on biological behavior of human ovarian cancer SKOV3 cells
10.16781/j.0258-879x.2017.06.0746
- Author:
Zheng HUANG
1
Author Information
1. Department of Obstetrics and Gynecology, Sixth People’s Hospital of Shanghai (South Branch), Shanghai Jiaotong University
- Publication Type:Journal Article
- Keywords:
Cell migration;
Cell proliferation;
LIM homeobox 8;
Neoplasm invasiveness;
Ovarian neoplasms
- From:
Academic Journal of Second Military Medical University
2017;38(6):746-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of LIM homeobox gene-8 (Lhx8) on the proliferation, metastasis and invasion of human ovarian cancer SKOV3 cells. Methods Lhx8-overexpression lentivirus (LV-Lhx8) was transfected into ovarian cancer SKOV3 cells to establish Lhx8-overexpression model and the negative control lentivirus (LV-NC) was used as control. Then Lhx8-siRNA and FAM-siRNA (used as control; NC group) siRNA was transfected into SKOV3 cells using Lipofectamine 2000 to construct cell interference model. Cells in wild type (WT) group were only given equivalent transfection reagent without virus or any interference fragment. The expression of Lhx8 was detected by immunofluorescence, qPCR and Western blotting. The proliferation of cells after overexpressing or interfering Lhx8 was measured by EDU assays and cell cycle assay. The migration and invasion of cells after transfection were measured by wound scratch experiments and Transwell assay. Results The expression of Lhx8 in SKOV3 cells in the LV-Lhx8 group was significantly higher than that in the LV-NC and WT groups (P<0.01), and its mRNA and protein expressions were significantly decreased after interfering Lhx8 (P<0.01). Compared with the WT and LV-NC groups, the proliferation of SKOV3 cells was significantly decreased in the LV-Lhx8 group and was significantly increased in the Lhx8-siRNA group (P<0.01). The cell cycle assay showed that Lhx8 overexpression significantly inhibited cell proliferation by increasing the number of cells in the G0/G1 phase, while the number of cells in the S phase in the Lhx8-siRNA group was significantly higher than that in the WT and NC groups (P<0.01). The migration and invasion of SKOV3 cells and the expression of matrix metalloproteinase (MMP)-2 and MMP-9 in the LV-Lhx8 group were significantly lower than those in the WT and LV-NC groups (P<0.01), while those in the Lhx8-siRNA group were significantly higher (P<0.01). Conclusion Lhx8 can inhibit the proliferation, migration and invasion of ovarian cancer SKOV3 cells, and down-regulate the expressions of MMP-2 and MMP-9.
