Effect of autophagy on expression of synaptophysin and postsynaptic density material 95 in CA1 hippocampal region of vascular dementia rats
10.16781/j.0258-879x.2017.02.0206
- Author:
Bin LIU
1
Author Information
1. First Department of Neurology, Affiliated Hospital of North China University of Science and Technology
- Publication Type:Journal Article
- Keywords:
Autophagy;
Postsynaptic density material 95;
Synaplophysin;
Synaptic plasticity;
Vascular dementia
- From:
Academic Journal of Second Military Medical University
2017;38(2):206-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of autophagy on the expression of synaptophysin (Syn) and postsynaptic density material 95 (PSD-95) in CA1 hippocanipal region of vascular dementia rats. Metluxls A total of 96 healthy male SD rals were randomly divided into sham operation group (Sham group)-vascular dementia model group (VD group)-autophagy inhibitor 3-mcthyl adenine preconditioning group (3-MA group) and autophagy agonist rapamycin preconditioning group (Rap group). Each group was further randomized into 4 subgroups 1, 2, 4 and 8 weeks after model establishment. The rat model of vascular dementia was established using a modified Pulsinelli four-vessel occlusion method. The expressions of Syn and PSD 95 in CA1 hippocanipal region of the vascular dementia rals were delected by Western blotting analysis. Results (1) Compared with the Sham group- the ratio of LC3- II LC3 I in CA1 hippocanipal region of the vascular dementia rats was significantly increased and the expressions of Syn and PSD-95 were significantly decreased in VD group at different time points (all P
