Generation and identification of carbohydrate response element-binding protein conditional gene knockout mouse model
10.16781/j.0258-879x.2019.02.0142
- Author:
Hao LI
1
Author Information
1. Department of Pathophysiology, College of Basic Medical Sciences, Naval Medical University (Second Military Medical University)
- Publication Type:Journal Article
- Keywords:
Carbohydrate response element-binding protein;
Fructose metabolism;
Gene targeting;
Knockout mice;
Lipid metabolism
- From:
Academic Journal of Second Military Medical University
2019;40(2):142-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a conditional knockout mouse model of transcription factor carbohydrate response element-binding protein (ChREBP), so as to provide a technical approach for exploring the biological function of ChREBP in vivo. Methods and results Using Cre/loxP gene targeting strategy, we constructed the gene targeting vector and franked the 8th exon of ChREBP gene with loxP site in embryonic stem cells (ES cells) via homologous recombination. We microinjected the recombined ES clones into blastocysts and implanted into the uterus of pseudopregnant mice to obtain the chimeric mice and ChREBPflox/+ mice. By crossbreeding ChREBPflox/+ mice with Alb-Cre mice, we generated liver-specific ChREBP knockout mice. Conclusion The generation of ChREBP conditional knockout mouse model provides an important means to reveal the physiological function and pathological significance of ChREBP in different tissues.
