Caries activity test by realtime PCR method and bacterial cultural method
10.11149/jkaoh.2020.44.3.126
- Author:
Joung-Hee YUN
1
;
Ji-Hyeon PARK
;
Ja-Won CHO
;
Sung-Won KIM
Author Information
1. Department of Oral Health, Graduate School of Public Health and Social Welfare, Dankook University, Cheonan, Korea
- Publication Type:Original Article
- From:Journal of Korean Academy of Oral Health
2020;44(3):126-129
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Objectives:This study aimed to compare the results of conventional methodology and real-time polymerase chain reaction (PCR) for acid-producing bacteria and identify the appropriate method for caries activity testing.
Methods:Oral examination and caries activity tests, such as the CRT® Bacteria test and modified Snyder test, were conducted on 33 middle school students in Cheongju, South Korea. Pearson correlation analysis was subsequently performed with a significance level of 5%.
Results:The amount of Streptococcus mutans determined using the CRT® Bacteria test was highly correlated with the decayed teeth (DT) index (R=0.570, P<0.05); decayed, missing, and filled teeth (DMFT) index (R=0.376, P<0.05); and dental health capacity of the first permanent molar (R=―0.395, P<0.05). The amount of Streptococcus mutans determined using real-time PCR was significantly associated with the DT index (R=0.528, P<0.05), DMFT index (R=0.369, P<0.05), and dental health capacity of the first permanent molar (R=―0.426, P=0.013).
Conclusions:There was a significant correlation between the amount and the type of bacteria between the CRT® Bacteria test and real-time PCR; therefore, these tests were found to be more appropriate to determine caries activity than the modified Snyder test. This study suggests that realtime PCR is a better technique for detecting caries activities than conventional techniques, such as the CRT® Bacteria test and modified Snyder test, because it is easy to use and provides accurate results.
