Determination of the K-value of different types of povidone by microfluidic rheometry and the relationship between K-value and weight-average molecular weight
10.16438/j.0513-4870.2020-0722
- VernacularTitle:微流体流变仪法测定不同型号聚维酮K值及K值与重均分子量关系的初步探讨
- Author:
Li HU
1
;
Jue WANG
1
;
Hui-min SUN
2
;
Jia-sheng TU
3
Author Information
1. Center for Research Development and Evaluation of Pharmaceutical Excipients and Generic Drugs, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients, National Institutes for Food and Drug Control, Beijing 100050, China
2. NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients, National Institutes for Food and Drug Control, Beijing 100050, China
3. Center for Research Development and Evaluation of Pharmaceutical Excipients and Generic Drugs, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
- Publication Type:Research Article
- Keywords:
povidone;
italic>K-value;
Ubbelohde capillary viscometer;
microfluidic rheometer;
gel permeation chromatography-multi angle laser light scattering;
eight-average molecular weight
- From:
Acta Pharmaceutica Sinica
2020;55(10):2454-2459
- CountryChina
- Language:Chinese
-
Abstract:
italic>K-values of 56 batches of 7 types of povidone were measured by microfluidic rheometry and with a Ubbelohde capillary viscometer. The K-values of the two methods were tested by SPSS software and the results showed that there was no significant difference between the two methods (P > 0.05). Taking K-values measured with the Ubbelohde capillary viscometer (Ku) as the abscissa and K-values measured by microfluidic rheometry (Km) as the ordinate a linear equation was calculated: Km = 0.893 9Ku + 4.617 6, R2 = 0.986 2, with good linearity, indicating that the microfluidic rheometer method can replace the Ubbelohde capillary viscometer in determining K-values of povidone. The microfluidic rheometer method has the benefits of less sample consumption, faster determination, and is more accurate, and it can be used with high-throughput automatic acquisition, which provides a more convenient method for the determination of K-values of different types of povidone. The weight-average molecular weights (Mw) of each type of povidone were measured by gel permeation chromatography-multi angle laser light scattering (GPC-MALLS), and the relationship between Mw and Km was lgMw = -0.000 4 Km2 + 0.072 7 Km + 2.791, R2 = 0.990 1. The fitting relationship was good, and Mw could be calculated by Km by the equation.
