Study on Regulation Effects of Volatile Oil from Angelica sinensis on Autophagy of Myocardial Cell H 9C2 in Rats with Hypoxia/reoxygenation Injury
- VernacularTitle:当归挥发油对缺氧/复氧损伤大鼠心肌细胞H9C2自噬的调控作用研究
- Author:
Yanpeng PU
1
;
Jiaming ZHOU
2
Author Information
1. Outpatient Department One of TCM，the First Affiliated Hospital of Jinzhou Medical University，Liaoning Jinzhou 121000，China
2. Dept. of Rehabilitation Medicine，Longwan District of Huludao Municipa l Central Hospital，Liaoning Huludao 125003，China
- Publication Type:Journal Article
- Keywords:
Volatile oil from Angelica sinensis;
Hypoxia/reoxygenation;
Myocardial cell H 9C2 of rats;
Autophagy
- From:
China Pharmacy
2020;31(20):2492-2497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To investigate the regulation effects of volatile oil from Angelica sinensis on autophagy of myocardial cell H 9C2 in rats with hypoxia and reoxygenation （H/R）injury. METHODS ：Using myocardial cell H 9C2 as subject ，CCK-8 method was used to screen the optimal concentration and administration time of volatile oil from A. sinensis. The acitivity of LDH in cell supernatant was determined after treated with volatile oil from A. sinensis by ELISA. Using autophagy inhibitors （3-methyladenine，5 mmol/L） as positive control ，MDC method and Western blotting assay were used to detect average fluorescence intensity of MDC and the expression of autophagy related proteins [Beclin- 1，microtubule associated protein light chain 3Ⅱ（LC3Ⅱ），LC3Ⅰ] in H 9C2 cells after treated with medicines. RESULTS ：After treated with 0.6 μmol/L violate oil from A. sinensis for 6 h，compared with blank group ，LDH activity in cell supernatant ，average fluorescence intensity of MDC ，the expression of Beclin- 1，LC3Ⅱ/LC3Ⅰ ratio in cells were increased significantly in H/R group ，while the expression of p 62 was decreased significantly （P＜0.05 or P＜0.01）. Compared with H/R group ，the activity of LDH in cell supernatant of H/R+drug group as well as average fluorescence intensity of MDC ，the expression of Beclin- 1，LC3Ⅱ/LC3Ⅰratio in cells in H/R+drug group and H/R+autophagy inhibitor group were decreased significantly ，while the expression of p 62 were increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：The volatile oil from A. sinensis can reduce the autophagy level of H/R injury myocardial cells by regulating the expression of autophagy related proteins.
