Characteristics of exosomes secreted by -infected mouse dendritic DC2.4 cells.
10.12122/j.issn.1673-4254.2020.05.19
- Author:
Dongliang LI
1
;
Shujun YANG
2
;
Hongjuan PENG
1
Author Information
1. Department of Pathogen Biology, School of Public Health, Southern Medical University. Guangzhou 510515, China.
2. Department of Ultrasound Diagnosis, 74th Army Group Hospital, Guangzhou 510318, China.
- Publication Type:Journal Article
- Keywords:
DC2.4 cells;
Toxoplasma gondii;
exosomes;
small RNA
- MeSH:
Animals;
Cell Line;
Dendritic Cells;
Exosomes;
Mice;
MicroRNAs;
RNA, Small Interfering;
Toxoplasma
- From:
Journal of Southern Medical University
2020;40(5):727-732
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes in the exosomes secreted by mouse dendritic cell line DC2.4 after infection with and to analyze the possible regulatory mechanisms underlying such changes.
METHODS:The exosomes were extracted by ultracentrifugation from DC2.4 cells at 28 h after infection with . The morphology of the exosomes was examined with transmission electron microscopy, and the exosome size and density were determined using a nanoparticle tracker. High-throughput sequencing was carried out to identify the differentially expressed small RNAs in the exosomes derived from the infected cells.
RESULTS: infection resulted in a significantly increased density of exosomes secreted by DC2.4 cells. Small RNA sequencing revealed that infection caused an increase in the number of miRNAs and piRNAs in the exosomes. The significantly up-regulated piRNAs after the infection included piR-mmu-159, piR-mmu-1526, piR-mmu-9082, piR-mmu-17405, and piR-mmu-25576.
CONCLUSIONS: infection causes accumulation and enrichment of exosomes secreted by DC2.4 cells with increased miRNAs and piRNAs in the exosomes.
