Investigation of modulating effect of Qingfei Paidu Decoction on host metabolism and gut microbiome in rats.
10.19540/j.cnki.cjcmm.20200609.201
- Author:
Gao-Song WU
1
;
Jing ZHONG
2
;
Ning-Ning ZHENG
1
;
Chao-Ran WANG
3
;
Hong-Li JIN
3
;
Guang-Bo GE
1
;
Jing-Yan HAN
4
;
Yue GAO
5
;
Li-Li SHENG
1
;
Wei-Dong ZHANG
6
;
Hou-Kai LI
1
Author Information
1. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
2. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University Huzhou 313000, China.
3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian 116023, China Innovation Institute of Medicine, China Medical City, Dalian Institute of Chemical Physics, Chinese Academy of Sciences Taizhou 225316, China.
4. Department of Integrated Traditional Chinese and Western Medicine, Peking University Health Science Center Beijing 100191, China.
5. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences Beijing 100039, China.
6. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China School of Pharmacy, Second Military Medical University Shanghai 200433, China.
- Publication Type:Journal Article
- Keywords:
COVID-19;
Qingfei Paidu Decoction;
gut microbiome;
immune;
inflammation;
metabolomics
- MeSH:
Animals;
Bacteria;
classification;
Chromatography, Liquid;
Drugs, Chinese Herbal;
pharmacology;
Gastrointestinal Microbiome;
drug effects;
Metabolomics;
Rats;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(15):3726-3739
- CountryChina
- Language:Chinese
-
Abstract:
This study is to explore the effect of Qingfei Paidu Decoction(QPD) on the host metabolism and gut microbiome of rats with metabolomics and 16 S rDNA sequencing. Based on 16 S rDNA sequencing of gut microbiome and metabolomics(GC-MS and LC-MS/MS), we systematically studied the serum metabolites profile and gut microbiota composition of rats treated with QPD for continued 5 days by oral gavage. A total of 23 and 43 differential metabolites were identified based on QPD with GC-MS and LC-MS/MS, respectively. The involved metabolic pathways of these differential metabolites included glycerophospholipid metabolism, linoleic acid metabolism, TCA cycle and pyruvate metabolism. Meanwhile, we found that QPD significantly regulated the composition of gut microbiota in rats, such as enriched Romboutsia, Turicibacter, and Clostridium_sensu_stricto_1, and decreased norank_f_Lachnospiraceae. Our current study indicated that short-term intervention of QPD could significantly regulate the host metabolism and gut microbiota composition of rats dose-dependently, suggesting that the clinical efficacy of QPD may be related with the regulation on host metabolism and gut microbiome.
