Effect of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma extracts on intestinal flora of vascular aging mice induced by high glucose and high lipid.
10.19540/j.cnki.cjcmm.20200117.401
- Author:
Jia-Li WANG
1
;
Cheng-Kui XIU
1
;
Jing YANG
1
;
Xue WANG
1
;
Yan-Hong HU
1
;
Jing-Yi FANG
2
;
Yan LEI
3
Author Information
1. Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Disease,Medical Experiment Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Guangdong Research Center for Integrative Medicine in Metabolic Diseases, Chinese Medicine Research Institute of Guangdong Pharmaceutical University Guangzhou 510006, China.
3. Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Disease,Medical Experiment Center, China Academy of Chinese Medical Sciences Beijing 100700, China Guangdong Research Center for Integrative Medicine in Metabolic Diseases, Chinese Medicine Research Institute of Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
extracts of Ginseng Radix et Rhizoma,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma;
high-glucose and high-fat;
intestinal flora;
vascular aging
- MeSH:
Aging;
Animals;
Drugs, Chinese Herbal;
Gastrointestinal Microbiome;
Glucose;
Lipids;
Mice;
Panax
- From:
China Journal of Chinese Materia Medica
2020;45(12):2938-2946
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to observe the changes of intestinal flora in vascular aging mice, in order to explore the relationship between vascular aging and intestinal flora and the effects of extracts of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on intestinal flora of vascular aging mice. A model of vascular aging in mice was induced through intrape-ritoneal injection with streptozotocin(STZ) combined with high-fat diet. Biochemical detection was performed on serum cholesterol(CHO), triglyceride(TG), high-density liptein cholesterol(HDL-C), low-density liptein cholesterol(LDL-C) and blood glucose(GLU). HE staining was used to detect mice thoracic aorta morphology, and the expressions of cyclin-dependent kinase inhibitor 2 A(p16) and cyclin-dependent kinase inhibitor 1 A(p21) protein in mice thoracic aorta were detected by Western blot. The 16 S rDNA gene of mice intestinal flora was detected by Illumina MiSeq high-throughput sequencing technology to explore the changes of intestinal flora in each group. The results demonstrated that the GLU level in low-dose and high-dose TCM groups decreased, but with unobvious changes in blood lipid indexes. Metformin could significantly decrease the levels of GLU(P<0.01), CHO and LDL-C in mice(P<0.05). Intravascular injury was not obvious in each drug group, and the expressions of p16 and p21 protein were significantly decreased(P<0.05). The intestinal flora of each group was mainly composed of Firmicutes(F) and Bacteroidetes(B) at the level of the phylum, but the B/F ratio was different from that of the youth group and the blank control group. The B/F ratio of the model group was significantly lower(P<0.01), and compared with the model group, the B/F ratio of the high-dose group and the metformin group was signi-ficantly higher(P<0.05). There were dominant and differential floras in the intestine of each group of mice. The results showed that extracts of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma could improve the intestinal flora structure and create a good intestinal environment by increasing the B/F ratio, which provides a new possible pathway for lowering blood glucose and blood lipids and delaying vascular aging.
